Kodi Elisa Kit amakambirana chiyani pankhani ya chithandizo ndi kusungidwa

Masitepe atatu amawonjezedwa mu Cida: fanizo, enzyme embzha, ndi gawo lapansi. Mukawonjezera zitsanzo, zowonjezera ziyenera kuwonjezeredwa pansi pa dzenje la Elisa Kit Kit, kuti lisatheze kumbali ya khoma la bowo, ndipo samalani kuti musawaze, osati kuwira. Zithunzi zimawonjezeredwa ndi microssuble, malinga ndi kuchuluka kwa kabowo. Mphuno yowiritsa iyenera kusinthidwa nthawi iliyonse yomwe fanizoli imawonjezedwa kuti isadetse mtanda. Thumba lolemera la pulasitiki limatha kugwiritsidwanso ntchito kuwonjezera zitsanzo.

Zochitika zotsatirazi zimaganiziridwa mu mankhwalawa ndi kuteteza zida za Elisa:

1, chidwi chiyenera kulipidwa kuti chiteteze. Hemoglobin ili ndi magulu a heme, omwe ali ndi zomwezi ndi peroxides. Chifukwa chake, mu Elisa Kit ndi HRP monga chizindikiro cha enzyme, ngati chiwonetsero cha hemoglobin mu seramu ndi chokwera, chimangokhala ndi gawo lolimba pokonzanso za HRP.

2. Pa nthawi yosonkhanitsa komanso kulekanitsidwa, muyenera kulipidwa kuti aletse kuipitsidwa ndi bakiteriya kuti athe. Ma enzy ena omwe amasungidwa ndi mabakiteriya amathanso kusiyanasiyana pa antiginies, ma antibodies ndi mapuloteni ena akakula. Kumbali inayo, ma enzymentate a ma enzymer michere, monga -galactidatidase a Escherheria Coli, chidzalowerera mobwerezabwereza pogwiritsa ntchito enzyme yofananira ngati chizindikiro.

3. Ngati seramu fanizoli limalekanitsidwa ndi ntchito ya Aseptic, imatha kusungidwa pa 2 ~ 8 digiri kwa sabata limodzi. Ngati ikugwiritsidwa ntchito ndi mabakiteriya, tikulimbikitsidwa kuti mukhale ozizira. Zitsanzozo ziyenera kusungidwa pansipa -70 digiri kwa nthawi yayitali.

4. Kuyang'anira kuyenera kulipidwa kwa Thaw Freen-Thaw komwe kumachitika chifukwa cha kulephera kwamphamvu kwa okhazikika a seramu seramu. Kuthamanga mobwerezabwereza kumawononga mphamvu ya fanizoli kudzawononga mapuloteni ndi mamolekyulu ena mu fanizoli, lomwe limapangitsa kuti Elisa Kit. Kuphatikiza apo, chidwi chiyeneranso kuperekera kusakanikirana kwa zoyesazi zakuthambo. Kugwedezeka mwankhanza sikuyenera kuchitika, ndipo kusakaniza kuyenera kubwerezedwanso kokha.

5. Pakakhala ku Turbidity kapena maluwa oyambitsidwa ndi kuipitsidwa ndi mabakiteriya poteteza, zoperewera ziyenera kutengedwa pambuyo pophatikizidwa.